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Hot Start PCR Reagents and Kits

In hot start PCR, key reagents are prevented from reacting in the PCR mixture until optimal temperatures are met. This reduces non-specific DNA amplification that occurs naturally at lower temperatures, increasing product yield, specificity, and sensitivity.
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1 – 15 of 187 results
1

Invitrogen™ Platinum™ Taq DNA Polymerase

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

EDGE ENCOMPASS_PREFERRED
Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Hot Start Built-In Hot Start
Overhang 3'-A
Starting Material DNA
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
2

Invitrogen™ Platinum™ Direct PCR Universal Master Mix
GREENER_CHOICE

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Polymerase Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Size (Final Product) 8 kb
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Universal Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang 3'-A
Green Features Less hazardous, less waste
Concentration 2X
For Use With (Application) Hot-start PCR,Real Time PCR (qPCR)
3

Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Hot Start Built-In Hot Start
Concentration 2X
For Use With (Application) Hot-start PCR
4

Thermo Scientific™ Phire Hot Start II PCR Master Mixes

Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
5

Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

EDGE
Shipping Condition Dry Ice
Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 2X
For Use With (Application) Hot-start PCR
6

Thermo Scientific™ Phire Hot Start II DNA Polymerase

Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format Separate Components
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
7
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Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase

Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

ENCOMPASS EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Polymerase Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type Hot Start DNA Polymerase
Reaction Speed Fast or Standard
Overhang 3'-A
Concentration 2X
8

Promega GoTaq™ G2 Hot Start Colorless Master Mixes

GoTaq™ G2 Hot Start Master Mixes are ready-to-use mixes containing GoTaq™ G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium

Content And Storage −30° to −10°C
Sample Type Colorless master mix
9

Invitrogen™ PCR Master Mix Starter Pack

The PCR Master Mix Starter Pack is composed of three different PCR master mixes for easy evaluation of their suitability for use in your research.

ENCOMPASS_PREFERRED
10

Invitrogen™ Platinum™ Taq Green Hot Start DNA Polymerase

Invitrogen Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase with a 10X Green PCR Buffer.

ENCOMPASS_PREFERRED
11

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

EDGE ENCOMPASS_PREFERRED
Polymerase AmpliTaq Gold DNA Polymerase
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Product Type DNA Polymerase
Overhang 3'-A
Concentration 5 U/μL
Exonuclease Activity 5' - 3'
12

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

EDGE ENCOMPASS_PREFERRED
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Product Type DNA Polymerase With Buffer I
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
13

Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

EDGE
Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Hot-start PCR
14

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

EDGE ENCOMPASS_PREFERRED
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 5 U/μL
Exonuclease Activity 5' - 3'
15

Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Wet or Dry Ice
Polymerase AccuPrime GC-Rich DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2X
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR